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. 2008 Oct 17;74(24):7514–7521. doi: 10.1128/AEM.01854-08

FIG. 3.

FIG. 3.

Transformation of a K. marxianus ura3 mutant with a linear DNA of S. cerevisiae URA3 (ScURA3). (A) The K. marxianus ura3 mutant was not transformed with the intact ScURA3 plasmid (pRS316) but with SmaI-digested pRS316 (4.8 kb) and the ScURA3 PCR fragment (1.7 kb). (B) Southern blot hybridization of chromosomal DNA of S. cerevisiae BY4704 (lane 1), K. marxianus wild-type (WT) DMKU3-1042 (lane 2), ura3 mutant RAK3605 (lane 3), and nine ScURA3 transformants from strain RAK3605 (lanes 4 to 12). Chromosomal DNA was digested with BamHI, run on a 0.8% agarose gel, transferred to a nylon membrane, and hybridized with a digoxigenin-labeled ScURA3 probe. The bands indicated by an arrowhead are likely the authentic K. marxianus URA3 that cross-hybridizes with ScURA3. M, DNA size marker.