FIG. 5.

Construction of an α-amylase expression strain in K. marxianus by transformation with a linear DNA. An ScTDH3 promoter-driven TAA (Aspergillus oryzae α-amylase gene) was constructed at the URA3 locus of S. cerevisiae and the PCR-amplified construct was then used without additional manipulation to transform a K. marxianus ura3 mutant. The appearance of clear zones around colonies indicates that the α-amylase gene was expressed under the control of the S. cerevisiae TDH3 promoter in K. marxianus.