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. 2008 Oct 24;74(24):7536–7545. doi: 10.1128/AEM.00894-08

FIG. 1.

FIG. 1.

BdbC-dependent secretion of PhoA by B. subtilis ItrxA. (A) Alkaline phosphatase activity was assayed using the growth media of B. subtilis 168(pPSPhoA5) (PhoA), B. subtilis ItrxA(pPSPhoA5) grown in the presence of 500, 100, or 25 μM IPTG (ItrxA 500, ItrxA 100, and ItrxA 25, respectively), B. subtilis bdbC(pPSPhoA5) (bdbC), and B. subtilis ItrxA bdbC(pPSPhoA5) grown in the presence of 25 μM IPTG (bdbC ItrxA 25). B. subtilis 168 (168) was used as a negative control. All strains were grown overnight in LB medium at 37°C. (B) Western blot analysis of TrxA production in B. subtilis ItrxA and parental strain 168. Cells were grown overnight at 37°C in LB medium. Cellular proteins were separated by SDS-PAGE, and TrxA was visualized by Western blotting. The IPTG concentrations in the medium (0, 25, 100, and 500 μM) are indicated above the blots. (C) Secretion of AmyQ by B. subtilis ItrxA. Cells of B. subtilis ItrxA(pKTH10) (ItrxA) and B. subtilis 168(pKTH10) (168) were grown in LB medium at 37°C in the presence of 25 μM IPTG. The presence of AmyQ in growth medium fractions was analyzed by SDS-PAGE and Western blotting.