TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Relevant properties | Reference or source |
|---|---|---|
| Plasmids | ||
| pMutin2mcs | pBR322-based integration vector for B. subtilis; contains a multiple cloning site downstream of the Pspac promoter and a promoterless lacZ gene; Apr Emr | 44 |
| pUC18 | Apr | 45 |
| pDG1727 | pMTL23 derivative; Apr Spr | 12 |
| pTOPO | pCR-Blunt II-TOPO vector; Kmr | Invitrogen Life Technologies |
| pXTC | pX derivative containing a tetracycline resistance marker instead of a chloramphenicol resistance marker; Apr Tcr | 7 |
| pXTC-ScdsbA | pXTC carrying dsbA of S. carnosus fused to the signal sequence and ribosome binding site of mntA of B. subtilis under transcriptional control of the xylA promoter; Apr Tcr | This study |
| pXTC-SadsbA | pXTC carrying dsbA of S. aureus fused to the signal sequence and ribosome binding site of mntA of B. subtilis under transcriptional control of the xylA promoter; Apr Tcr | 18 |
| pPSPhoA5 | Plasmid carrying the E. coli phoA gene fused to the pre-pro region of the lipase gene from S. hyicus; Cmr | 7 |
| pKTH10 | Encodes AmyQ of B. amyloliquefaciens; Kmr | 29 |
| B. subtilis strains | ||
| 168 | trpC2 | 21 |
| ItrxA | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the trxA gene; trxA-lacZ Emr Pspac-trxA; IPTG dependent | 34 |
| ybdE | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the ybdE gene; ybdE-lacZ | 16 |
| ydfQ | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the ydfQ gene; ydfQ-lacZ | 16 |
| ydbP | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the ydbP gene; ydbP-lacZ | 16 |
| ykuV | trpC2 ykuV::Spr (MunI-NgoMIV integration) | This study |
| ytpP | trpC2 ytpP::Spr (BsaAI integration) | This study |
| yusE | trpC2 yusE::Spr (BclI-BclI integration) | This study |
| stoA (ykvV) | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the stoA gene; stoA-lacZ | 16 |
| yneN | Derivative of 168; contains an integrated copy of plasmid pMutin2 in the yneN gene; yneN-lacZ | 16 |
| SPβ | trpC2; ΔSPβ; sublancin 168 sensitive; laboratory name, CBB312 | 8 |
| bdbC | trpC2 bdbC::Kmr | 8 |
| ItrxA bdbC | trpC2 trxA::pMutin2 bdbC::Kmr; IPTG dependent | This study |
| X-ScdsbA | trpC2; amyE::XTC-ScdsbA | This study |
| ItrxA X-ScdsbA | trpC2; Pspac-trxA; amyE::XTC-ScdsbA | This study |
| X-SadsbA | trpC2; amyE::XTC-SadsbA; also known as XdsbA | 18 |
| ItrxA X-SadsbA | trpC2; Pspac-trxA; amyE::XTC-SadsbA | This study |
| S. carnosus TM300 | Source of dsbA gene used in the present study | 35 |
| S. aureus RN4220 | Restriction-deficient derivative of NCTC 8325, cured of all known prophages | 19 |
| E. coli DH5α | supE44 hsdR17 recA1 gyrA96 thi-1 relA1 | 13 |