TABLE 1.
Genes involved in FA metabolism in Y. lipolytica and S. cerevisiaea
| Gene | S. cerevisiae name | EC no. | Y. lipolytica ortholog | % Amino acid identityb (% gap) | Function |
|---|---|---|---|---|---|
| GUT1 | YHL032c | EC 2.7.1.30 | YALI0F00484g | 35 (29) | Glycerol kinase |
| GPD1 | YDL022w | EC 1.1.1.18 | YALI0B02948g | 52 (11) | G3P dehydrogenase (NAD+) |
| GPD2 | YDL059w | EC 1.1.1.18 | 61 (11) | G3P dehydrogenase (NAD+) | |
| GUT2 | YIL155c | EC 1.1.99.5 | YALI0B13970g | 44 (11) | G3P dehydrogenase |
| SCT1 | YBL011w | EC 2.3.1.15 | YALI0C00209g | 40 (7) | G3P acyltransferase |
| GPT2 | YKR067w | EC 2.3.1.15 | 28 (7) | G3P acyltransferase | |
| SLC1 | YDL052c | EC 2.3.1.51 | YALI0E18964g | 33 (23) | 1-Acyl-sn-G3P acyltransferase |
| DGA1 | YOR245c | EC 2.3.1.20 | YALI0E32769g | 33 (27) | DAG acyltransferased |
| LRO1 | YNR008w | EC 2.3.1.158 | YALI0E16797g | 45 (4) | Phospholipid:DAG acyltransferased |
| TGL3 | YMR313c | EC 3.1.1.3 | YALI0D17534g | 21 (13) | Triacylglycerol lipase |
| TGL4 | YKR089c | EC 3.1.1.3 | YALI0F10010g | 29 (13) | Triacylglycerol lipase |
| TGL5 | YOR081c | EC 3.1.1.3 | 29 (13) | Triacylglycerol lipase | |
| ARE1 | YCR048w | EC 2.3.1.26 | YALI0F06578g | 30 (7) | Acyl-CoA:sterol acyltransferasec |
| ARE2 | YNR019w | EC 2.3.1.26 | 33 (7) | Acyl-CoA:sterol acyltransferase | |
| TGL1 | YKL140w | EC 3.1.1.13 | YALI0E32035g | 32 (11) | Cholesterol esterase |
| POX1 | YGL205w | EC 6.2.1.3 | YALI0E32835g | 35 (10) | Acyl-CoA oxidasec |
| POX2 | YALI0F10857g | 35 (8) | Acyl-CoA oxidased | ||
| POX3 | YALI0D24750g | 35 (9) | Acyl-CoA oxidased | ||
| POX4 | YALI0E27654g | 33 (0) | Acyl-CoA oxidasec | ||
| POX5 | YALI0C23859g | 35 (0) | Acyl-CoA oxidasec | ||
| POX6 | YALI0E06567g | 35 (2) | Acyl-CoA oxidasec | ||
| MFE1 | YKR009c | EC 4.2.1.74 | YALI0E15378g | 49 (5) | Multifunctional β-oxidation proteinc |
| POT1 | YIL160c | EC 2.3.1.16 | YALI0E18568g | 55 (4) | Peroxisomal oxoacyl thiolasec |
Shown are genes, corresponding S. cerevisiae gene names and EC (Enzyme Commission) numbers, Y. lipolytica orthologs (gene name), and corresponding functions. Bioinformatic data were obtained from the Saccharomyces Genome Database (http://www.yeastgenome.org/) and the Genolevures database (http://cbi.labri.fr/Genolevures/).
The comparative analysis was based on pairwise alignments of the full protein sequence predicted by the ClustalX program (http://bips.u-strasbg.fr/fr/Documentation/ClustalX/), and sequence identity or similarity was determined on the basis of this full alignment with Genedoc (http://www.nrbsc.org/gfx/genedoc/). ID, identity. % gap, percentage of residues unaligned within gaps.
Confirmed by functional analysis.
Confirmed by protein characterization.