TABLE 2.
E. coli and Y. lipolytica strains and plasmids used in this study
| Strain (host strain) | Genotype or plasmid | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | φ80 dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Promega |
| JME459 (DH5α) | pBluescript II KS+ (ColE1 ori LacZ bla) | Stratagene |
| JME461 (DH5α) | pRRQ2 (cre ARS68 LEU2 in pBluescript II KS+) | 12 |
| JME508 (DH5α) | 1.6-kb I-SceI fragment containing hph marker, MH cassette | 12 |
| JME507 (DH5α) | 1.3-kb I-SceI fragment containing URA3 marker, MU cassette | 12 |
| JME743 (DH5α) | 1.6-kb PCR fragment containing ylGUT2PT cassette in pCR4Blunt-TOPO | This work |
| JME641 (DH5α) | 1.3-kb PCR fragment containing ylLEU2PT cassette in pDrive | This work |
| MTLE34 (DH5α) | 1.6-kb PCR fragment containing ylPOX1PT cassette in pBluescript KS+ | This work |
| MTLE35 (DH5α) | 1.6-kb PCR fragment containing ylPOX6PT cassette in pBluescript KS+ | This work |
| JME744 (DH5α) | MU cassette in JME743, ylGUT2PUT cassette | This work |
| JME651 (DH5α) | MH cassette in JME641, ylLEU2PHT cassette | This work |
| MTLE36 (DH5α) | MU cassette in MTLE34, ylPOX1PHT cassette | This work |
| MTLE37 (DH5α) | MU cassette in MTLE35, ylPOX6PHT cassette | This work |
| Y. lipolytica strains | ||
| W29 | MATa WT | 1 |
| Po1d | MATaura3-302 leu2-270 xpr2-322 | |
| JMY330 | MATaleu2-270 xpr2-322 | This work |
| MTLY40 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 | 21 |
| MTLY64 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 leu2::hph | This work |
| MTLY66 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 Δleu2 | This work |
| MTLY82 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 Δleu2 pox1::hph | This work |
| MTLY85 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 Δleu2 Δpox1 | This work |
| MTLY94 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 Δleu2 Δpox1 pox6::hph | This work |
| MTLY97 | MATaura3-302 xpr2-322 Δpox2 Δpox3 Δpox4 Δpox5 Δleu2 Δpox1 Δpox6 | This work |
| JMY1202 | MATaura3-302 leu2-270 xpr2-322 Δgut2::URA3 | This work |
| JMY1351 | MATaura3-302 xpr2-322 Δleu2 Δpox1-6 Δgut2::URA3 | This work |