FIG. 2.

TLC analysis of phospholipids. (A) Proteinase K (1 U/ml; 1 ml) was added to the BacMP suspension (final concentration, 1 mg/ml). After being washed, BacMPs were suspended in chloroform-methanol (2:1) to extract the lipid membrane. The lipid fraction was finally dissolved with chloroform and subjected to TLC analysis (lane 3). The sample obtained from nontreated BacMPs was also subjected as a control (lane 2). PE was used as a standard (lane 1). (B) BacMPs treated with proteinase K were further treated with PLD (lane 4). Nontreated BacMPs (lane 3), PE (lane 1), and phosphatidic acid (lane 2) were used as a control and standards. All samples were applied to a silica gel plate with chloroform-methanol-water (65:25:4, vol/vol/vol) and detected by Dittmer reagent.