TABLE 5.
Hemolytic activity of each peptide against goat erythrocytesa
| Peptide | % Hemolytic activity at:
|
|
|---|---|---|
| 1 h | 4 h | |
| 6-7/2 | 0.0 ± 1.0 | 1.5 ± 1.3 |
| 6-7/3 | 0.0 ± 0.6 | 1.5 ± 1.9 |
| 6-7/5 | 0.0 ± 0.5 | 2.0 ± 2.0 |
| 6-7/7 | 0.0 ± 0.5 | 0.0 ± 1.4 |
Goat erythrocytes (2%) were incubated with 100 μM peptides at 37°C. The absorbance increase by hemoglobin release in the supernatant was evaluated. The increase of A550 by the addition of 1% Triton X-100 was defined as 100%. Triplicate measurements for each experiment were performed.