TABLE 2.

Oligonucleotides used as primers

Primer name	Primer sequence	Location (bp)	Use
C427	5′-CTACATTACGCATTTGGA-3′	533-516a	iPCR
Tn917F	5′-TGGTCTTAAGGCAGCCAGAG-3′	2180-2199a	iPCR
Tn4431	5′-CCATCAAGGGTATGAGTA-3′	4537-4520a	iPCR
Tn5054	5′-AAATAGATCCCGAAGTAAC-3′	5143-5161a	iPCR
Tn4292	5′-TTGAGCACGAGTCAGAGC-3′	4292-4275a	Southern blot probe
Tn3768	5′-AACAATATTCGATCAGGAGA-3′	3767-3786a	Southern blot probe
InsL	5′-AGCCATCAAAGAAAGGAAGA-3′	1794567-1794586b	Amplification of insertion site
InsR	5′-GTATCGGTGAAAGGCAACAG-3′	1795615-1795596b	Amplification of insertion site
LM DBR-F	5′-CGCTCAAACCAATTTCTGCTA-3′	1794114-1794134b	RT-PCR
LM DBR-R	5′-CTTCCTTTCTTTGATGGCTTT-3′	1794585-1794565b	RT-PCR
16S rRNA-F	5′-TTAGTGGCGGACGGGTGAGT-3′	rrsA-rrsB	RT-PCR
16S rRNA-R	5′-CGGACAACGCTTGCCACCTA-3′	rrsA-rrsB	RT-PCR
P1	5′-GTCCGCCTTAATAGAGCA-3′	1794392-1794409b	Identification of revertant
P2	5′-GTTCTACCGCTGGCTCTT-3′	1797225-1797208b	Identification of revertant
C1	5′-GGGCGTCTCAGCTCTAAAATCCG-3′	4385-4406a	Identification of allelic exchange
C2	5′-TAGGAACTATGAAAAAGGAACGGA-3′	1729-1706a	Identification of allelic exchange
M13fwd	5′-GGTTTTCCCAGTCACGAC-3′		Identification of allelic exchange
M13rev	5′-AGCGGATAACAATTTCACAC-3′		Identification of allelic exchange

^aLocation in the published nucleotide sequence of Tn917 (accession number M11180).

^bLocation in the published complete genome of L. monocytogenes strain 4b F2365 (accession number AE017262).