TABLE 4.
Primers used in multiplex amplifications for the MLGT assays
| Lineage(s) | Region | Primer | Sequence (5′-3′)a |
|---|---|---|---|
| II-III-IV | VGCa | hly-F2 | GAAGGAGAGTGAAACCCATG |
| II-III-IV | VGCa | mpl-R | CTCGCAACTTCYGGCTCAGC |
| II-III-IV | VGCb | actA-F | CCAAYTGCATTACGATTAACC |
| II-III-IV | VGCb | plcB-R | CAAGCACATACCTAGAACCAC |
| II-III-IV | INLa | inlA-F2 | ACGAAYGTAACAGACACGGTC |
| II | INLa | inlA-R2 | CTACTTCTATTTACTAGCACG |
| III-IV | INLa | inlA-R3 | TCTAGCTCTTYACACTACTTC |
| II-III-IV | INLb | inlB-F2 | AATCAAGGAGAGGATAGTGTG |
| II-III-IV | INLb | inlB-R | CTACCGGRACTTTATAGTAYG |
| II | LMO | lmo0300-F | GATTGGATTAAGTACGAGC |
| II | LMO | lmo0298-R | CTCTTATCAATGTGAAGGTGC |
| III-IV | LMO | lmo0300-F2 | ATGTRGCGAGCGATCACTACC |
| III-IV | LMO | lmo0300-R2 | AATCTTCGTGRTAGTGTTTGC |
| II | TRP | adhE-F | TGGAAGTTTGAACCATTGCAT |
| II | TRP | adhE-R | AGGACGAGAGACCCGTGGTA |
| II | ACC | dnaE-F | GGATTTTCDCTTGGAGAAGCAG |
| II | ACC | dnaE-R | CRCCGACAACAGARCCCATAC |
a
See IUPAC codes for definitions of degenerate bases.