TABLE 5.
ASPE probes and probe performance data
| Probeb | Probe sequencec | Adjusted MFI (range)
|
Target Nd | IDe | |
|---|---|---|---|---|---|
| Nontarget | Target | ||||
| Lm2.ACC1 (94) | CTTTCTATCTTTCTACTCAATAATccgttatgtttccggaatgtc | 7-183 | 1,943-2,340 | 1 | 10.6 |
| Lm2.ACC2 (11) | TACAAATCATCAATCACTTTAATCggtattatgacaaaatgtccg | 44-158 | 2,379-3,043 | 14 | 15.1 |
| Lm2.ACC3 (67) | TCATTTACTCAACAATTACAAATCaagaagaaagcggatgtcctg | 104-231 | 2,916-2,935 | 1 | 12.6 |
| Lm2.ACC4 (63) | CTACTTCATATACTTTATACTACAggggatagtaaggttatcgtt | −3 to 193 | 3,424-4,285 | 4 | 17.8 |
| Lm2.ACC5 (55) | TATATACACTTCTCAATAACTAACgacgattttggcaactattgatgt | 48-208 | 3,949-3,974 | 1 | 19.0 |
| Lm2.ACCx (69) | CTATAAACATATTACATTCACATCgtttcagcgcayccagtatcg | N/Aa | 3,118-4,842 | 125 | N/A |
| Lm2.INLa1 (5) | CAATTCAAATCACAATAATCAATCcaccaccttccgcaaatatag | 266-545 | 5,880-6,371 | 1 | 10.8 |
| Lm2.INLa10 (13) | CAATAAACTATACTTCTTCACTAAaacagatggkaaaaaatg | 60-63 | 1,399-3,002 | 124 | 22.3 |
| Lm2.INLa11 (98) | AATCATACTCAACTAATCATTCAAaaacaggyggaactaaatggrat | 56-73 | 320-1,569 | 123 | 4.4 |
| Lm2.INLa12 (8) | AATCCTTTTACATTCATTACTTACgcaaatgacattacgctgtag | 16-165 | 2,063-2,806 | 17 | 12.5 |
| Lm2.INLa13 (59) | TCATCAATCAATCTTTTTCACTTTgtgcgctttcaggtttaactagtctat | 95-254 | 2,390-2,882 | 3 | 9.4 |
| Lm2.INLa14 (65) | CTTTTCATCAATAATCTTACCTTTcatttagtggaactgtgacgt | 22-127 | 2,172-2,508 | 4 | 17.2 |
| Lm2.INLa15 (97) | AATCTCATAATCTACATACACTATaaacaggyggaactaaatggat | 27-97 | 1,981-2,380 | 2 | 20.5 |
| Lm2.INLa2(54) | CTTTTTCAATCACTTTCAATTCATgcaccactgtcgggtctaacg | 143-307 | 2,436-2,645 | 1 | 7.9 |
| Lm2.INLa3 (28) | CTACAAACAAACAAACATTATCAAcgaaaaaacagatgggaaaaaata | 17-133 | 981-1,048 | 1 | 7.4 |
| Lm2.INLa4 (23) | TTCAATCATTCAAATCTCAACTTTccttatatgcccaatatagcgct | 41-246 | 4,533-4,839 | 1 | 18.4 |
| Lm2.INLa5 (74) | TACACATCTTACAAACTAATTTCAcggatataacgccacttaaag | 20-108 | 852-1,417 | 39 | 7.9 |
| Lm2.INLa6 (12) | TACACTTTCTTTCTTTCTTTCTTTaactaatctacagcaattatt | −13 to 72 | 487-582 | 1 | 6.7 |
| Lm2.INLa7 (100) | CTATCTTTAAACTACAAATCTAACgcaaatgayattacgctgtac | 54-100 | 1,194-2,157 | 108 | 12.0 |
| Lm2.INLa8 (70) | ATACCAATAATCCAATTCATATCAgtgcgctttcaggtttaaytartctac | 52-82 | 753-1,542 | 122 | 9.2 |
| Lm2.INLa9 (43) | CTTTCAATTACAATACTCATTACAcatttagtggaacygtgacgc | 39-59 | 1,103-2,309 | 121 | 18.6 |
| Lm2.INLax (62) | TCAATCATAATCTCATAATCCAATccaaataagtaacatcagtc | N/A | 906-1,751 | 125 | N/A |
| Lm2.INLb1 (29) | AATCTTACTACAAATCCTTTCTTTgatgttgatggaacggtaatt | 47-202 | 2,129-2,331 | 1 | 10.5 |
| Lm2.INLb2 (60) | AATCTACAAATCCAATAATCTCATggatagtgtgaaagaaaagcacg | 18-125 | 678-838 | 1 | 5.4 |
| Lm2.INLb3 (20) | CTTTTACAATACTTCAATACAATCcttccacgagaagataactca | 72-197 | 4,568-4,847 | 1 | 23.1 |
| Lm2.INLb4 (73) | ATCAAATCTCATCAATTCAACAATccgaaaaagcagtcaacttaact | 66-185 | 4,930-5,110 | 1 | 26.6 |
| Lm2.INLb5 (72) | TCATTTACCTTTAATCCAATAATCcaagcggagactatcaccgtgt | 45-115 | 2,249-2,804 | 22 | 19.5 |
| Lm2.INLb6 (18) | TCAAAATCTCAAATACTCAAATCAcgtatcttgcgcgaagccaaaacg | 128-242 | 2,391-3,030 | 14 | 9.9 |
| Lm2.INLbx (24) | TCAATTACCTTTTCAATACAATACgtacaagcggagactatcacc | N/A | 1,315-2,072 | 125 | N/A |
| Lm2.LMO1 (26) | TTACTCAAAATCTACACTTTTTCAactcggattcgctataagcat | 68-269 | 5,230-5,319 | 1 | 19.5 |
| Lm2.LMO10 (86) | CTAATTACTAACATCACTAACAATcgaatgcagcacaaacgtcgg | 1-314 | 6,231-7,806 | 20 | 19.8 |
| Lm2.LMO11 (22) | AATCCTTTTTACTCAATTCAATCAcgtatctagcaagttttgatag | −28 to 826 | 8,534-10,203 | 8 | 10.3 |
| Lm2.LMO12 (14) | CTACTATACATCTTACTATACTTTtggcgttgctggctaagttta | 32-106 | 1,458-2,039 | 23 | 13.8 |
| Lm2.LMO2 (3) | TACACTTTATCAAATCTTACAATCgtgttgccagaagcggcttca | 44-179 | 1,945-2,045 | 1 | 10.9 |
| Lm2.LMO3 (30) | TTACCTTTATACCTTTCTTTTTACtcgtgcgattttgctagttct | 61-401 | 8,661-9,449 | 1 | 21.6 |
| Lm2.LMO4 (76) | AATCTAACAAACTCATCTAAATACcacaaacggctcaatatcaaa | 30-126 | 2,612-2,864 | 1 | 20.8 |
| Lm2.LMO5 (6) | TCAACAATCTTTTACAATCAAATCcaggcaagcaggaagccatta | 46-217 | 4,267-4,554 | 1 | 19.6 |
| Lm2.LMO6 (16) | AATCAATCTTCATTCAAATCATCAggagaccaagcgtagtagccg | 153-311 | 5,100-7,093 | 39 | 16.4 |
| Lm2.LMO7 (19) | TCAATCAATTACTTACTCAAATACggcagcatggacataaaggcg | 113-242 | 1,417-1,572 | 1 | 5.9 |
| Lm2.LMO8 (9) | TAATCTTCTATATCAACATCTTACttcgccagttcggcaaygaggtca | 27-388 | 2,770-7,807 | 12 | 7.1 |
| Lm2.LMO9 (37) | CTTTTCATCTTTTCATCTTTCAATcacaaccagcatcgattgcgtta | 46-168 | 2,866-3,809 | 9 | 17.1 |
| Lm2.LMOx (56) | CAATTTACTCATATACATCACTTTcgattaagccatcttgtaagcc | N/A | 2,368-4,315 | 125 | N/A |
| Lm2.TRP1 (77) | CAATTAACTACATACAATACATACgacaaatacccactatctcat | 219-781 | 3,431-5,879 | 3 | 4.4 |
| Lm2.TRP2 (88) | TTACTTCACTTTCTATTTACAATCgtgacatttataacggcttcatca | 524-930 | 10,830-11,262 | 1 | 11.6 |
| Lm2.TRP3 (93) | CTTTCTATTCATCTAAATACAAACcaagtaagaaaaaagaactacccg | 26-122 | 3,373-4,622 | 17 | 27.7 |
| Lm2.TRP4 (89) | TATACTATCAACTCAACAACATATcaaatgataattcgcttaaatct | 28-141 | 3,133-3,359 | 1 | 22.3 |
| Lm2.TRP5 (91) | TTCATAACATCAATCATAACTTACgcaacaagctaaatttttttggg | 100-234 | 2,937-3,000 | 1 | 12.6 |
| Lm2.TRPx (78) | CTATCTATCTAACTATCTATATCAcacatcatgtccgctactgac | N/A | 742-1,506 | 125 | N/A |
| Lm2.VGCa1 (80) | CTAACTAACAATAATCTAACTAACggagagtgaaaacccatgaaaaaat | 24-101 | 528-544 | 1 | 5.2 |
| Lm2.VGCa2 (96) | ATACTAACTCAACTAACTTTAAACctgaagcaaaggatgcatctgt | 14-118 | 770-931 | 1 | 6.5 |
| Lm2.VGCa3 (44) | TCATTTACCAATCTTTCTTTATACgcttttgatgctgccgtaagt | 60-134 | 1,935-2,033 | 1 | 14.4 |
| Lm2.VGCa4 (2) | CTTTATCAATACATACTACAATCAgagaaacaccaggagttcccg | 166-322 | 3,646-3,873 | 1 | 11.3 |
| Lm2.VGCa5 (21) | AATCCTTTCTTTAATCTCAAATCAggagatgcagtgacaaatgtgcca | 39-140 | 1,322-1,420 | 1 | 9.4 |
| Lm2.VGCa6 (51) | TCATTTCAATCAATCATCAACAATgcaaaacgtataatttagttct | 25-105 | 1,870-2,066 | 2 | 17.8 |
| Lm2.VGCa7 (66) | TAACATTACAACTATACTATCTACagcttgggaatggtggagaaa | 152-271 | 998-1,185 | 3 | 3.7 |
| Lm2.VGCa8 (57) | CAATATCATCATCTTTATCATTACagttgtaggtggcttaaactc | 15-82 | 365-587 | 18 | 4.4 |
| Lm2.VGCa9 (17) | CTTTAATCCTTTATCACTTTATCAgaccggaacctaccacttgta | 53-171 | 2,816-2,952 | 1 | 16.5 |
| Lm2.VGCax (40) | CTTTCTACATTATTCACAACATTAcactcagcattgatttgccagg | N/A | 721-2,016 | 125 | N/A |
| Lm2.VGCb1 (41) | TTACTACACAATATACTCATCAATtttgccagagacaccgatgca | 17-199 | 4,435-4,499 | 1 | 22.3 |
| Lm2.VGCb2 (10) | ATCATACATACATACAAATCTACAgacataactaaaaaagcgccattc | 24-129 | 3,518-4,056 | 1 | 27.3 |
| Lm2.VGCb3 (35) | CAATTTCATCATTCATTCATTTCAccgaccgaccagctatacaagta | 15-362 | 2,783-3,117 | 1 | 7.7 |
| Lm2.VGCb4 (1) | CTTTAATCTCAATCAATACAAATCgcaaacaggaaatgtggctac | 6-180 | 1,549-2,654 | 15 | 8.6 |
| Lm2.VGCb5 (85) | ATACTACATCATAATCAAACATCAcaccaaagctagcagaacttccta | 84-450 | 4,210-5,031 | 4 | 9.4 |
| Lm2.VGCbx (7) | CAATTCATTTACCAATTTACCAATgcagcgacagatagcgaaga | N/A | 1,241-3,935 | 125 | N/A |
| Lm3-4.INLa1 (76) | AATCTAACAAACTCATCTAAATACgcaccaccaacaactggagga | 74-477 | 3,026-3,861 | 2 | 6.3 |
| Lm3-4.INLa2 (19) | TCAATCAATTACTTACTCAAATACgctcaatatagcgccaatagctt | 33-199 | 2,294-2,484 | 1 | 11.5 |
| Lm3-4.INLa3 (74) | TACACATCTTACAAACTAATTTCAgcaacgtttgataatgacggtt | 55-252 | 2,932-3,403 | 1 | 11.6 |
| Lm3-4.INLa4 (88) | TTACTTCACTTTCTATTTACAATCgcaacctttgatgttgatggg | 357-683 | 5,316-8,397 | 15 | 7.8 |
| Lm3-4.INLa5 (95) | TACACTTTAAACTTACTACACTAAgcaacctttgatgttgatgga | 458-1,026 | 2,506-4,353 | 33 | 2.4 |
| Lm3-4.INLa6 (94) | CTTTCTATCTTTCTACTCAATAATgacatcgatttatatgcgct | 6-122 | 3,325-3,468 | 1 | 27.4 |
| Lm3-4.INLa7 (43) | CTTTCAATTACAATACTCATTACAtaaatcggctagaactatcc | 1-108 | 1,802-1,865 | 1 | 16.8 |
| Lm3-4.INLa8 (68) | TCATAATCTCAACAATCTTTCTTTgtcccctaacaggtctaact | 45-217 | 2,217-2,333 | 1 | 10.2 |
| Lm3-4.INLa9 (51) | TCATTTCAATCAATCATCAACAATcagctacacagcaacgttta | 18-150 | 1,402-1,521 | 1 | 9.4 |
| Lm3-4.INLax (12) | TACACTTTCTTTCTTTCTTTCTTTgtgatattagtgcgctttcagg | N/Aa | 2,634-5,197 | 48 | N/A |
| Lm3-4.INLb1 (17) | CTTTAATCCTTTATCACTTTATCAgcacgatttcatgggagagtaat | 153-481 | 3,418-4,144 | 1 | 7.1 |
| Lm3-4.INLb2 (29) | AATCTTACTACAAATCCTTTCTTTccttacaatacagccggct | −37 to 129 | 9,517-10,444 | 1 | 73.7 |
| Lm3-4.INLb3 (32) | ATTATTCACTTCAAACTAATCTACgaatatgacaaaggtgttactt | 42-211 | 2,417-2,589 | 1 | 11.5 |
| Lm3-4.INLb4 (72) | TCATTTACCTTTAATCCAATAATCcacttgtgaacaagctttct | 27-144 | 3,308-3,515 | 1 | 23.1 |
| Lm3-4.INLb5 (6) | TCAACAATCTTTTACAATCAAATCgaggcagggacgcgaataat | 179-445 | 2,074-2,270 | 1 | 4.7 |
| Lm3-4.INLb6 (37) | CTTTTCATCTTTTCATCTTTCAATggaatccagtatttacccc | −5 to 97 | 3,654-3,740 | 1 | 37.7 |
| Lm3-4.INLb7 (62) | TCAATCATAATCTCATAATCCAATctcgcaccgctgtaaagctc | 24-163 | 2,506-2,515 | 1 | 15.3 |
| Lm3-4.INLb8 (73) | ATCAAATCTCATCAATTCAACAATaacggggcgaaagtacaagcc | 49-177 | 3,231-3,515 | 1 | 18.2 |
| Lm3-4.INLbx (100) | CTATCTTTAAACTACAAATCTAACtatgcgaatattgcgcgaagcc | N/A | 2,051-3,476 | 48 | N/A |
| Lm3-4.LMO1 (20) | CTTTTACAATACTTCAATACAATCtttgcccaggatagccaccc | 28-540 | 4,538-6,339 | 5 | 8.4 |
| Lm3-4.LMO2 (2) | CTTTATCAATACATACTACAATCAgccgcgaacatcacaaccagt | 56-989 | 4,372-9,565 | 9 | 4.4 |
| Lm3-4.LMOx (59) | TCATCAATCAATCTTTTTCACTTTcgattaagccatcttgtaagcc | N/A | 2,238-6,590 | 48 | N/A |
| Lm3-4.VGCa1 (3) | TACACTTTATCAAATCTTACAATCcaggtgatgtagaattaacg | 62-277 | 3,464-3,693 | 1 | 12.5 |
| Lm3-4.VGCa2 (99) | AATCTACACTAACAATTTCATAACgcctaacatatccaggtgca | 33-178 | 2,823-3,013 | 1 | 15.9 |
| Lm3-4.VGCa3 (91) | TTCATAACATCAATCATAACTTACctttatccgaaatatagtaatagc | 17-331 | 2,417-2,718 | 1 | 7.3 |
| Lm3-4.VGCa4 (70) | ATACCAATAATCCAATTCATATCAgcatatcccaaagtttaagt | 83-227 | 4,419-5,184 | 1 | 19.4 |
| Lm3-4.VGCa5 (48) | AAACAAACTTCACATCTCAATAATgtaaaagaatgtctggcttta | 32-414 | 4,849-5,369 | 2 | 11.7 |
| Lm3-4.VGCa6 (64) | CTACATATTCAAATTACTACTTACattgatgaccggaacttaccg | 42-554 | 4,879-6,285 | 3 | 8.8 |
| Lm3-4.VGCa7 (42) | CTATCTTCATATTTCACTATAAACgaccggaacttaccacttgta | 52-309 | 3,452-3,651 | 1 | 11.2 |
| Lm3-4.VGCa8 (49) | TCATCAATCTTTCAATTTACTTACggagttcccattgcttatacaacc | 98-262 | 5,326-5,495 | 1 | 20.4 |
| Lm3-4.VGCa9 (26) | TTACTCAAAATCTACACTTTTTCAggaggctacgttgctcaattcaat | 620-1,652 | 9,110-9,702 | 1 | 5.5 |
| Lm3-4.VGCax (28) | CTACAAACAAACAAACATTATCAAgtataccacggagatgcagtgac | N/A | 498-1,304 | 48 | N/A |
| Lm3-4.VGCb1 (77) | CAATTAACTACATACAATACATACcaatcccaacagaagaagagg | 54-912 | 8,267-9,471 | 1 | 9.1 |
| Lm3-4.VGCb2 (14) | CTACTATACATCTTACTATACTTTcgtcatccaggattgccatca | 30-968 | 7,661-8,679 | 1 | 7.9 |
| Lm3-4.VGCb3 (57) | CAATATCATCATCTTTATCATTACgcttgaaagccttacttatctgt | 16-383 | 2,970-3,421 | 1 | 7.8 |
| Lm3-4.VGCb4 (35) | CAATTTCATCATTCATTCATTTCAgtttactagcagtccggttct | 5-139 | 2,991-3,505 | 3 | 21.5 |
| Lm3-4.VGCb5 (44) | TCATTTACCAATCTTTCTTTATACggtgtgttctctttaggggct | 97-336 | 2,475-2,997 | 2 | 7.4 |
| Lm3-4.VGCb6 (18) | TCAAAATCTCAAATACTCAAATCAgcaaaataagcgcaccggctt | −10 to 147 | 2,658-3,136 | 1 | 18.1 |
| Lm3-4.VGCb7 (97) | AATCTCATAATCTACATACACTATcaattgttgataaaagtgcaggga | 134-504 | 1,859-2,196 | 2 | 3.7 |
| Lm3-4.VGCb8 (30) | TTACCTTTATACCTTTCTTTTTACatgcttcggactttccacct | 59-1,071 | 10,699-11,430 | 1 | 10.0 |
| Lm3-4.VGCb9 (24) | TCAATTACCTTTTCAATACAATACgatatgccgagcctaccat | 89-459 | 4,224-4,322 | 1 | 9.2 |
| Lm3-4.VGCb10 (5) | CAATTCAAATCACAATAATCAATCgagcagccaagcgaggtaaac | 66-255 | 1,415-1,507 | 1 | 5.6 |
| Lm3-4.VGCb11 (16) | AATCAATCTTCATTCAAATCATCAagcgaggtaaatacgggaccg | −117 to 199 | 4,627-5,741 | 3 | 23.3 |
| Lm3-4.VGCb12 (46) | TACATCAACAATTCATTCAATACActagctgatttaagagatagg | 275-561 | 5,167-5,742 | 3 | 9.2 |
| Lm3-4.VGCb13 (80) | CTAACTAACAATAATCTAACTAACgaacaaactgagaatgcggcc | 12-182 | 6,160-7,025 | 1 | 33.9 |
| Lm3-4.VGCb14 (60) | AATCTACAAATCCAATAATCTCATcttcctatcacgaaagcacg | 22-208 | 2,986-3,392 | 1 | 14.3 |
| Lm3-4.VGCb15 (33) | TCAATTACTTCACTTTAATCCTTTagacttgctttgccagagacc | 97-612 | 5,032-5,259 | 1 | 8.2 |
| Lm3-4.VGCb16 (96) | ATACTAACTCAACTAACTTTAAACcataatatttgcaacgacagataa | 37-195 | 2,375-2,671 | 1 | 12.2 |
| Lm3-4.VGCb17 (55) | TATATACACTTCTCAATAACTAACcacagatgaatgggaagaagg | 103-315 | 4,468-4,926 | 1 | 14.2 |
| Lm3-4.VGCb18 (86) | CTAATTACTAACATCACTAACAATctggataaaccaacaaaagcaac | 7-148 | 3,477-3,936 | 1 | 23.6 |
| Lm3-4.VGCbx (50) | CAATATACCAATATCATCATTTACgacttagattctagcatgcagtc | N/A | 1,484-4,209 | 48 | N/A |
a
N/A, not applicable for positive control probes.
b
The 64 probes used in the lineage II assay are prefaced with Lm2, while the 51 probes used in the lineage III and IV assay are prefaced with Lm3-4. Luminex microsphere sets (Luminex Corporation) used for hybridization reactions are indicated in parentheses.
c
The 5′ sequence tag portions of extension probes are capitalized.
d
Target sample size among the 125 lineage II and 48 lineage III and IV strains sequenced.
e
ID, index of discrimination.