TABLE 6.
Mutations leading to truncated forms of InlA among 125 sequenced isolates from L. monocytogenes lineage II
Mutationa | Equivalentb | MLGT probe(s)c | MLGT haplotype(s) | RTE (%)d |
---|---|---|---|---|
T189 | Lm2.INLa8, Lm2.INLa13 | Lm2.8, Lm2.30 | 3.9 | |
T492 | H1 | Lm2.INLa9, Lm2.INLa14 | Lm2.20, Lm2.34, Lm2.38 | 3.9 |
T577 | LO28 | Lm2.INLa11, Lm2.INLa15 | Lm2.24 | 2.6 |
T685 | NV5 | Lm2.INLa10 | Lm2.21 | 0.0 |
T700 | PMSC3 | Lm2.INLa7, Lm2.INLa12 | Lm2.5, Lm2.19, Lm2.33 | 20.8 |
Mutation nomenclature reflects the amino acid position of premature stop codons relative to the 800-amino-acid protein predicted for strain EGD-e (NC_003210).
Strain names or previous designations for equivalent mutations described by Jonquieres et al. (19), Olier et al. (29), Rousseaux et al. (39), and Nightingale et al. (26).
Four of the five truncation mutations were targeted by pairs of reciprocal probes, one of which was specific to the nucleotide character state responsible for the truncation, while the other probe was specific to the alternate form of the inlA allele. After multiple attempts, only a single acceptable probe was identified for the T685 mutation.
Frequency of InlA truncation mutations among 77 lineage II isolates collected by USDA-FSIS from RTE products and processing facilities.