TABLE 2.
Transformation efficiencies of Bacillus cereus and Bacillus weihenstephanensis strainsa
| Recipient strain | Plasmid | Transformation efficiency (no. of CFU μg−1) of:
|
|||
|---|---|---|---|---|---|
| Nontreated cells | CFEDSM11821 | CFEB434 | CFEATCC 14579 | ||
| DSM11821 | pIL253 | NT | 3.8 × 103 | 1 × 102 | NT |
| None | 3 | 9.8 × 104 | 3 × 101 | ||
| B434 | pIL253 | NT | 8.0 × 103 | 4.8 × 103 | NT |
| None | 2.3 × 104 | ||||
| ATCC 14579 | pIL253 | 3.5 × 106 | 2.2 × 106 | ND | 1.5 × 106 |
| None | 7.5 × 105 | 2.3 × 106 | |||
| KBAB4 | pIL253 | 2.5 × 104 | 2.5 × 104 | ND | ND |
a
Plasmid pIL253 was in vitro methylated with cell extracts of strain DSM11821 (CFEDSM11821), strain B434 (CFEB434), or strain ATCC 14579 (CFEATCC 14579). Each value is derived from an independent experiment. NT, no transformants were obtained; ND, not determined.