TABLE 3.
Generation of large deletions in the telomere-neighboring region of the short arm of chromosome 3, including the AF gene cluster, by use of the A. sojae Δku70 strain
| Deletion length (kb) | 5-FOA frequencya | No. of strains
|
Efficiency (%) | |
|---|---|---|---|---|
| 5-FOA resistant | Large deletion | |||
| 30 (first half) | (1.9 ± 0.8) × 10−4 | 12 | 12 | 100 |
| 30 (second half) | (3.9 ± 4.1) × 10−6 | 10 | 10 | 100 |
| 60 (entire AF) | (2.2 ± 0.8) × 10−5 | 10 | 9 | 90 |
| 100 | (2.4 ± 1.3) × 10−7 | 8 | 2 | 25 |
| 200 | (2.3 ± 1.4) × 10−7 | 8 | 0 | 0 |
a
Frequency of occurrence of 5-FOA-resistant strains (number of colonies on 5-FOA plates/total number of colonies spread). Numbers are the means ± standard deviations for triplicate experimental data.