Skip to main content
. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Mol Cell. 2008 Sep 5;31(5):737–748. doi: 10.1016/j.molcel.2008.08.011

Figure 1. Thermodynamic analysis of the assembly of the gp130/LIF-R/CNTF-Rα/CNTF complex.

Figure 1

(A) Schematic representation of gp130, LIF-R, CNTF-Rα, and CNTF. IgD denotes “Ig-like domain”, while CHR denotes “cytokine-binding homology region” and FnIII denotes “Fibronectin-type III domain”. Each CHR contains three yellow lines to represent conserved disulfides and a WSXWS motif. (B) Isothermal titration calorimetry illustrating the assembly of CNTF and CNTF-Rα, followed by assembly of the CNTF-Rα/CNTF binary complex with gp130 minus or plus the D1 IgD. (C) Titration of LIF-R with CNTF, the CNTF-Rα/CNTF binary complex, and the gp130/CNTF-Rα/CNTF ternary complex. Inset tables list the thermodynamic parameters for the representative binding isotherms.