Table 1.
Potency of ATP-α-S, Sp and Rp isomers, at mutant P2Y11GFP receptors to increase intracellular calcium was determined as described in methods
| Receptor construct | Receptor expression level (%) |
EC50 values of ATP-α-S (μM) |
|
|---|---|---|---|
| Sp isomer | Rp isomer | ||
| Wild-type | 100 | 1.71±0.55 (4) | 0.27±0.06 (5) |
| Glu186Ala | 111±10 (9)a | 10.2±1.75 (6) | 0.85±0.11 (7) |
| Arg268Ala | 143±23 (3)a | 30.8±6.43 (5) | 26.4±6.28 (4) |
| Arg268Gln | 96±8 (7)a | 3.28±0.71 (4) | 2.69±0.63 (4) |
| Glu186Ala/ Arg268Gln | 94±6 (4) | 19.8±3.92 (4) | 13.7±3.32 (5) |
a
Data were taken from (Zylberg et al., 2007).
Data represent mean EC50 values (μM)±s.e.m. obtained from the concentration–response curves shown in Figure 1. We analysed 1321N1 cells stably expressing the wild-type or mutated receptor in (n) numbers of experiments. Receptor expression level was determined as described before (Zylberg et al., 2007).