Table 5.
Luminol enhanced whole blood chemiluminescence (LBCL) measured before and after polysomnography controlled sleep
| Patients undergoing polysomnography | |||||
| Chemiluminescence parameter | Time of the day | OSAS | Severe OSAS | CPAP-OSAS | Controls |
| rCL [aU/104p] | Evening | 2.4 ± 2.2 | 1.9 ± 1.8 | 2.2 ± 1.9 | 1.9 ± 1.8 |
| Morning | 2.3 ± 2.2 | 2.5 ± 2.8 | 1.5 ± 0.9 | 1.6 ± 1.5 | |
| Diff and 95% CI | 0.1 (-0.7 – 0.8) | 0.6 (0.15 – 1.02) | 0.7 (0.1 – 1.2) | 0.2 (-0.1 – 0.5) | |
| pCL [aU/104p] | Evening | 6.4 ± 5.1 | 4.9 ± 4.7 | 6.0 ± 4.4 | 5.5 ± 4.3 |
| Morning | 5.9 ± 4.6 | 5.9 ± 4.8 | 4.3 ± 2.9 | 4.7 ± 2.5 | |
| Diff and 95% CI | 0.5 (-0.9 – 1.8) | 0,9 (-0.4 – 2.2) | 1.6 (0.4 – 2.9) | 5.2 (-2,0 – 12.4) | |
| tCL [aU·s/104p] | Evening | 1939 ± 1532 | 1503 ± 1391 | 1805 ± 1278 | 1642 ± 1316 |
| Morning | 1790 ± 1371 | 1778 ± 1442 | 1313 ± 820 | 1416 ± 961 | |
| Diff and 95% CI | 149 (-275 – 573) | 275 (-117 – 668) | 492 (132 – 852) | 225 (22 – 430) | |
| Peak time [s] | Evening | 281 ± 31 | 276 ± 37 | 293 ± 30 | 270 ± 24 |
| Morning | 275 ± 30 | 277 ± 37 | 284 ± 22 | 339 ± 23 | |
| Diff and 95% CI | 6 (0.3 – 11.5) | 1 (-5.3 – 6.5) | 9 (0.1 – 16.3) | 69 (-22 – 160) | |
rCl – the average resting chemiluminescence prior to addition of fMLP; pCL – maximal chemiluminescence signal after addition of fMLP; tCl – total light emission after cell stimulation with fMLP; peak time – the time from fMLP addition to the appearance of pCL; OSAS – patients with the untreated OSAS; Severe OSAS – patients with AHI ≥ 30; CPAP-OSAS – patients with OSAS treated successfully after a first attempt with CPAP, controls – subjects without OSAS Results expressed as mean ± SD, difference of means with 95% confidence interval (Diff and 95% CI) No significant differences (p > 0.05) were found for evening and morning LBCL parameters within and between the studied groups.