Table 2.
Details of primers and amplicons for the 6 evaluated genes.
| Gene | Primer | Sequence (5' → 3') | Genomic Position | Amplicon Length | correlation coefficients (R2) | qRT-PCR Efficiency (%) |
| B2M | Forward | CTCCGTGGCCTTAGCTGTG | 1st Exon | 69 bp | 0.998 | 90.1 |
| Reverse | TTTGGAGTACGCTGGATAGCCT | 1st/2nd exon | ||||
| GAPDH | Forward | TGCACCACCAACTGCTTAGC | 7th Exon | 87 bp | 0.997 | 92 |
| Reverse | GGCATGGACTGTGGTCATGAG | 7th/8th exon | ||||
| HMBS | Forward | TGCAACGGCGGAAGAAAA | 1st/2nd Exon | 113 bp | 0.998 | 91.0 |
| Reverse | ACGAGGCTTTCAATGTTGCC | 3rd exon | ||||
| HPRT1 | Forward | TGACACTGGCAAAACAATGCA | 6th Exon | 94 bp | 0.997 | 94.9 |
| Reverse | GGTCCTTTTCACCAGCAAGCT | 6th/7th exon | ||||
| SDHA | Forward | TGGGAACAAGAGGGCATCTG | 2nd Exon | 86 bp | 0.996 | 87.8 |
| Reverse | CCACCACTGCATCAAATTCATG | 3rd exon | ||||
| UBC | Forward | CGGTGAACGCCGATGATTAT | 1st Exon | 124 bp | 0.994 | 88.4 |
| Reverse | ATCTGCATTGTCAAGTGACGA | 1st/2nd exon |