The degree of evolutionary change inferred by a range of phylogenetic methods compared with the simulated amount of evolutionary change under (a) strong, (b) mild and (c) no purifying selection. Filled triangles, MP; filled diamonds, Jukes Cantor (JC); filled squares, Hasegawa, Kishino Yano (HKY)+Γ; pluses, mixture model (MM); crosses, temporal hidden Markov model (THMM); open diamonds, equiprobable (EQU); open squares, Whelan and Goldman (WAG)+F+Γ; grey solid line, inferred equals simulated.