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Time-dependent release of TGF-β1 in fibroblast/platelet co-culture. Fibroblasts (HFL1) or fibroblasts together with platelets (Plt) were cast into collagen gels. After 2, 12, 24 or 48 hours of culture, the medium surrounding the gels were collected and analysed for TGF-β1 by ELISA. Vertical axis: concentration of TGF-β1 (pg/mL) in medium. Data shown as mean of duplicates ± SD.
