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. 2008 May 27;55(2):301–310. doi: 10.1111/j.1365-313X.2008.03505.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

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Binding of LeHB-1 to LeACO1 promoter fragments.

(a) Outlines of the LeHB-1 protein and GST-HD-Zip fusion protein used for DNA binding.

(b) Expression of fusion protein in yeast. Lane 1, GST-LeHB1 HD-Zip fusion; lane 2, GST; lane 3, 1 μg BSA.

(c) Structure of the LeACO1 promoter showing the three regions used for LeHB-1 binding (F4-1, F3-1 and F1-1).

(d)Comparison of AtHB-1 binding sequence with similar sequences found in F1-1, F4-1 and F3-1 of the LeACO1 promoter.

(e) Gel retardation assays with F1-1 and F4-1 fragments, and the LeHB-1 GST-HD-Zip fusion protein. Lane 1, free F1-1 probe; lane 2, GST-HD-Zip with F1-1 probe; lane 3, GST with F1-1 probe; lane 4, sample from lane 2 plus a 200-fold molar excess of unlabelled competitor F1-1; lane 5, free F4-1 probe; lane 6, GST-HD-Zip with the F4-1 probe; lane 7, GST with the F4-1 probe; lane 8, sample from lane 6 plus a 200-fold molar excess of unlabelled competitor F4-1.