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. 2009 Jan 16;5(1):e1000268. doi: 10.1371/journal.ppat.1000268

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Ferrari et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Fluconazole susceptibility testing of DSY562 pdr1Δ mutant strain (SFY93) expressing different CgPDR1 alleles, which were named according to their strain number origin and by indicating the amino acid substitution (in superscript) associated with a specific strain number. The following strains correspond to the indicated genotypes: DSY562 pdr1Δ+486:SFY98; 489^L328F: SFY99; 738: SFY100; 739^R376W: SFY101; 2253: SFY102; 2254^D1082G:SFY103; 2235: SFY104; 2234^T588A: SFY105; 701: SFY106; 704^T607S: SFY107; 529:SFY108; 530^E1083Q: SFY109; 753: SFY110; 754^Y584C: SFY111; 726: SFY112; 727^D876Y: SFY113; BPY55^P882L: SFY116. (B) Expression of CgCDR1, CgCDR2, and CgSNQ2 in the DSY562 pdr1Δ mutant strain (SFY93) expressing different CgPDR1 alleles, named according to their strain number origin. Quantification was performed by real-time RT-PCR. The values, which are averages of four separated experiments, represent the increase in gene expression relative to DSY562 (set at 1.00). Error bars show standard deviations.