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. 2009 Jan 16;5(1):e1000268. doi: 10.1371/journal.ppat.1000268

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Ferrari et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) In vitro fitness assays. Strains SFY114 (DSY562 pdr1Δ-PDR1) and SFY115 (DSY562 pdr1Δ-L280F) were inoculated in YEPD in single or mixed (1∶1) cultures. Aliquots were taken from each culture in triplicate and population ratios were calculated from the proportion of azole-susceptible and azole-resistant colonies plated onto YEPD agar as described in Material and Methods. (B) In vivo fitness assays. SFY114 (DSY562 pdr1Δ-PDR1) and SFY115 (DSY562 pdr1Δ-L280F) were inoculated intravenously as single or mixed (1∶1) cultures to three groups of mice as described in Material and Methods. Kidneys were taken at given time points from sacrificed mice and population ratios were measured as described above.