Table 3.

MLPA Results of CVS (Case 7) with STC 46,XY and LTC 47,XY,+18 Using Three Different Sample Preparation Methods

Sample preparation method	Mean probe ratios (95% CI) fraction C	Mean probe ratios (95% CI) fraction M	Mean probe ratios (95% CI) fraction T
Whole villi proteinase K treatment	-	-	#13: 0.91 (0.83−0.99)
			#18: 1.18 (1.08−1.28)
			#21: 0.92 (0.85−0.99)
			#X: 0.51 (0.47−0.56)
			#Y: 0.45 (0.35−0.54)
Enzymatic dissociation	#13: 1.00 (0.90−1.10)	#13: 0.82 (0.77−0.87)	#13: 0.96 (0.86−1.06)
(coll, tryp/EDTA)	#18: 1.00 (0.86−1.14)	#18: 1.44 (1.35−1.54)	#18: 1.16 (1.01−1.30)
	#21: 1.02 (0.89−1.14)	#21: 0.80 (0.75−0.86)	#21: 0.90 (0.81−1.00)
	#X: 0.49 (0.41−0.58)	#X: 0.45 (0.43−0.47)	#X: 0.49 (0.42−0.57)
	#Y: 0.51 (0.45−0.58)	#Y: 0.42 (0.33−0.50)	#Y: 0.47 (0.37−0.57)
Enzymatic dissociation	#13: 0.94 (0.74−1.13)	#13: 0.84 (0.75−0.93)	#13: 0.86 (0.81−0.91)
(tryp/EDTA, coll)	#18: 0.92 (0.80−1.05)	#18: 1.41 (1.26−1.57)	#18: 1.37 (1.30−1.44)
	#21: 1.17 (0.99−1.36)	#21: 0.81 (0.75−0.88)	#21: 0.82 (0.78−0.86)
	#X: 0.59 (0.39−0.78)	#X: 0.44 (0.39−0.49)	#X: 0.46 (0.41−0.51)
	#Y: 0.60 (0.31−0.90)	#Y: 0.43 (0.33−0.53)	#Y: 0.45 (0.36−0.54)

^#, chromosome; tryp, trypsin; coll, collagenase.