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. 2008 Nov 20;27(24):3235–3245. doi: 10.1038/emboj.2008.242

Figure 5.

Figure 5

Model of dynein, Lis1 and CLIP-170 function in bipolar spindle assembly. Dynein binds along the lattice of spindle microtubules together with Lis1 near the zone of antiparallel microtubule overlap. When a growing microtubule plus-end, decorated with CLIP-170, passes the dynein complex, CLIP-170 can interact with the dynein complex, possibly through its interaction with Lis1. Minus-end-directed movement of dynein now pulls the two centrosomes together (left). In contrast, when a CLIP-115-decorated microtubule plus-end passes the dynein complex, it does not interact with Lis1 and no minus-end-directed force is produced (middle). When Eg5 is inhibited, a complex of dynein, Lis1 and CLIP-170 pulls centrosomes together, resulting in monopolar spindle formation (right).