Table 1.

Results from average in-gel FRET and single-molecule FRET (smFRET) measurements

Cy3 site	RNA	X-ray model (1Y1W)	In-gel FRET	Distance in-gel FRET, Å	Single-molecule FRET	Distance smFRET, Å
Rpb3	GE2	64 Å*	0.34, 1.11 R0	64	0.40 (28%), 1.07 R0	64
					0.49 (72%), 1.01 R0	60
	GE9	NA	0.28, 1.17 R0	68	0.32 (83%), 1.13 R0	68
					0.42 (17%), 1.06 R0	63
	GE18	NA	0.16, 1.32 R0	77	0.18 (67%), 1.29 R0	77
					0.51 (33%), 0.99 R0	59
Rpb4	GE2	82 Å*	0.18, 1.29 R0	80	0.17 (>95%), 1.3 R0	78
	GE9	NA	0.39, 1.08 R0	67	0.18 ( 9%), 1.29 R0	77
					0.30 (91%), 1.15 R0	69
	GE18	NA	0.78, 0.81 R0	50	0.29 (13%), 1.16 R0	70
					0.63 (87%), 0.92 R0	55
DNA	GE2	50 Å*	0.51, 0.99 R0	50	0.44, 1.05 R0	50
	GE9	NA	0.38, 1.08 R0	55 (60 ± 5)†	0.34, 1.13 R0	54 (60 ± 5)†
	GE18	NA	0.16, 1.32 R0	67 (65 ± 5)‡	Close to noise	NA (65 ± 5)‡

R₀ of in-gel FRET, 58 Å (Rpb3); 62 Å (Rpb4); 50 Å (DNA); R₀ of smFRET: 60 Å (Rpb3, Rpb4); 48 Å (DNA).

*The distance is measured from X-ray model (1Y1W).

^†Predicted by triangulation based on Rpb3–GE9 and Rpb4–GE9.

^‡Predicted by triangulation based on Rpb3–GE18 and Rpb4–GE18.