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. Author manuscript; available in PMC: 2009 Feb 26.
Published in final edited form as: Biochemistry. 2008 Jan 24;47(8):2638–2649. doi: 10.1021/bi702235k

Figure 2.

Figure 2

Fluorescence emission spectra of WALP23 and of DHE in bilayers of DMPC containing different concentrations of sterols at pH 7.0 and 30 °C. (A) The solid lines are spectra from FRET experiments using increasing DHE concentrations (from top to bottom, 0, 0.25, 0.5, 1, 2, 4, and 8 mol %) added to the lipid fraction. The dashed lines show the fluorescence emission of the tryptophans of WALP23, obtained by decomposition. (B) Fluorescence emission of DHE (from top to bottom, 8, 4, 2, 1, 0.5, and 0.25 mol %), obtained by decomposition of the spectra in panel A. (C) Samples as in panel A but with equimolar amounts of DHE and cholesterol added to the lipid fraction. (D) Fluorescence emission of DHE in the presence of equimolar amounts of cholesterol, obtained by decomposition of the spectra in panel C. In all samples, the excitation wavelength was 280 nm and the final concentration of WALP23 was 5 μM using a peptide/lipid ratio of 1/200.