Figure 1.

Expression of nAChR, NMDAR, AMPAR, GABA_AR and GlyR transcripts in skeletal muscle during normal development. RT-PCR was used for detection of subunit transcripts of five neurotransmitter receptors in muscle, notochord and neural tube at three stages of development. Tissue-specific RNA was analysed from embryos (a) at 1 day (stage 22), (b) at 1.3 days (stage 28) and from larvae (c) at 3 days (stage 40). Primers were designed from predicted Xenopus sequences for nAChRα1, NR1, GluR1, GABA_ARβ2 and GlyRα1 subunits and for neuronal markers NeuroD and Neurogenin-related protein-1 (NGNR-1). EF-2, elongation factor 2. Reproduced with permission from Borodinsky & Spitzer (2007).