Figure 9.

Movements of single-headed myosin VI. (a) Time course of displacement of single-headed myosin VI (i) without bead and (ii) with bead. The traces in (i) were measured by the fluorescence position of GFP fused with myosin head and the trace in (ii) was measured by the bead position. (b) Stepping model for cargo-bound single-headed myosin VI. Huge cargo keeps the myosin head near the actin filament and the head repeatedly attaches and detaches to several actin monomers during diffusion. Finally, the head preferentially lands on the forward binding site.