Fig. 7.

Spt16 association with SLACS, but not the SL RNA gene. ChIP was performed with antibodies directed against the BB2 epitope present on Spt16 or Pob3, and precipitates were analysed by PCR with primers specific for the regions indicated (Ab). A control with no antibody (no Ab) and an aliquot of the total input (input) is also shown. The double bands observed at SLACS regions 1 and 3 are a consequence of sequence heterogeneity, i.e. nucleotide deletions/insertions, in the 5′ UTR and ORF2, respectively, of SLACS elements.