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. 2008;61:51–59. doi: 10.3114/sim.2008.61.04

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Copyright © Copyright 2008 CBS Fungal Biodiversity Centre

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Fig. 1. — Determination of the ApOLE1 (A), ApODE12 (B) and ApELO1 (C) gene copies in A. pullulans. Southern blotting of genomic DNA digested with different restriction endonucleases (EcoRI, HindIII, BamHI, EcoRI+HindIII, as indicated). Southern blots of the gels with separately digested DNAs were probed with radiolabelled fragments, and amplified with oligonucleotide primers specific for parts of the genes in question.