Table 1.

Specific stages of development of the pupae are marked by distinct frequency and amplitude heartbeat properties.

stage (%D)	BB	max principal frequency (Hz)	r.m.s. amplitude (μm)
0–50	1	0.52±0.03	2.66±0.15
50–75	2	0.54±0.02	7.56±2.95
75–83	3	0.64±0.04	13.31±1.07
83–92	erratic beating	0.72±0.03	17.33±0.23
92–100	constant beating	0.95±0.02	26.43±0.87