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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):148–153. doi: 10.4049/jimmunol.182.1.148

FIGURE 2. Suppressor potential of Tregs.

FIGURE 2

(a) Naïve CD4+T cells isolated from the LN of unprimed BALB/c recipients and (b) allo-primed CD4+T cells isolated from the LN of allograft rejectors were stimulated with CD3-antibody for 3 days in the presence of different Tregs isolated from the LN of syngeneically grafted recipients, allograft acceptors, and allograft rejectors at week 3 post-transplantation. The activity of Tregs is measured at Treg:Teff cell ratio of 1:2. Proliferation was measured using the BrdU incorporation assay and compared with the proliferative responses of respective CD3-stimulated naïve or allo-primed T cell in the absence of Tregs and % suppression was calculated as described in Materials and Methods. Each transplant group consists of 4-6 recipient mice. Data from a representative experiment of three performed is shown. P values are calculated using student’s t-test and error bars represent SEM. *p = 0.018; §p = 0.0002.