A, Immunoblot analysis of caspase-8, BID, and β-actin in cell lysates following the transfection with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h. The asterisk (*) represents a non-specific protein that is recognized by the capase-8-specific antibody. B, Cells were transfected with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h, stained with 10 μM of Hoechst 33342 and 500 nM MitoTracker Red, and visualized by fluorescent microscopy. C, Immunoblot analysis of caspase-9, caspase-7, caspase-6, and β-actin in cell lysates following the transfection of MCF-7 with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h.