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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2008 Sep 17;76(12):1694–1704. doi: 10.1016/j.bcp.2008.09.007

Fig. 2. c-FLIP knockdown induces the activation of caspases, Bid, and mitochondrial disintegration.

Fig. 2

A, Immunoblot analysis of caspase-8, BID, and β-actin in cell lysates following the transfection with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h. The asterisk (*) represents a non-specific protein that is recognized by the capase-8-specific antibody. B, Cells were transfected with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h, stained with 10 μM of Hoechst 33342 and 500 nM MitoTracker Red, and visualized by fluorescent microscopy. C, Immunoblot analysis of caspase-9, caspase-7, caspase-6, and β-actin in cell lysates following the transfection of MCF-7 with non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT) for 48 h.