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. Author manuscript; available in PMC: 2009 Dec 15.

Published in final edited form as: Biochem Pharmacol. 2008 Sep 17;76(12):1694–1704. doi: 10.1016/j.bcp.2008.09.007

Fig. 4 — A, Immunoblot analysis of FADD and β-actin in cell lysates following the transfection with 100 nM of non-targeting siRNA (SC) or FADD-specific siRNA for 72 h. B, Immunoblot analysis of caspase-8 and β-actin in cell lysates treated with 100 nM FADD-specific siRNA for 24 h, and treated for an additional 48 h with 100 nM of non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT). The asterisk (*) represents a non-specific protein that is recognized by the capase-8-specific antibody. C, The density of the caspase-8-p18 band in the c-FLIP-specific siRNA (FT) or FADD siRNA and FT siRNA treated cell lysates was quantified using the Image J 1.37c software (values +/− s.d.). D, Cells were treated with 100 nM FADD-specific siRNA for 24 h and treated for an additional 48 h with 100 nM non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT), and apoptosis was determined by counting fragmented nuclei after staining with Hoecsht 33342 among 200 cells. All values are representative of three independent experiments, and error bars show standard deviation from triplicate counts.

Fig. 4 — A, Immunoblot analysis of FADD and β-actin in cell lysates following the transfection with 100 nM of non-targeting siRNA (SC) or FADD-specific siRNA for 72 h. B, Immunoblot analysis of caspase-8 and β-actin in cell lysates treated with 100 nM FADD-specific siRNA for 24 h, and treated for an additional 48 h with 100 nM of non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT). The asterisk (*) represents a non-specific protein that is recognized by the capase-8-specific antibody. C, The density of the caspase-8-p18 band in the c-FLIP-specific siRNA (FT) or FADD siRNA and FT siRNA treated cell lysates was quantified using the Image J 1.37c software (values +/− s.d.). D, Cells were treated with 100 nM FADD-specific siRNA for 24 h and treated for an additional 48 h with 100 nM non-targeting siRNA (SC) or c-FLIP-specific siRNA (FT), and apoptosis was determined by counting fragmented nuclei after staining with Hoecsht 33342 among 200 cells. All values are representative of three independent experiments, and error bars show standard deviation from triplicate counts.