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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):572–580. doi: 10.4049/jimmunol.182.1.572

Figure 7. IL-23-IL-17-G-CSF axis mediates HS/R-induced CD11b+/Gr-1+ cells mobilization from BM.

Figure 7

Animals were injected intraperitoneally with one of the neutralizing antibodies against IL-23 (100 μg per mouse), IL-17 (100 μg per mouse), G-CSF (100 μg per mouse) or nonimmune control IgG, or a combination of anti-G-CSF Ab and anti-IL-23 Ab, or anti-G-CSF Ab and anti-IL-17 Ab 10 min before hemorrhage. Femoral bones and whole blood from the mice were harvested at 4 h after HS/R or sham operation, and cells in BM and blood were then collected for detection of CD11b+/Gr-1+ cells by flow cytometry. Representative density plots of CD11b+/Gr-1+ cells in BM (A) and blood (B) are shown. The graph depicts the mean and SE of the changes in CD11b+/Gr-1+ cells in BM and blood from three mice. * P< 0.01 as compared with sham groups.