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GC/MS analysis of cell walls of M. smegmatis, M. smegmatisΔaftC, M. smegmatisΔaftC pMV261-Ms-aftC and M. smegmatisΔaftC pMV261-Mt-aftC. Samples of per-O-methylated cell walls were hydrolysed with 2 M TFA, reduced, per-O-acetylated and analysed as described under Experimental procedures (Besra et al., 1995; Alderwick et al., 2005).
