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RT-PCR showing expression of T3SS1 genes and secY. cDNA (+) made from total RNA isolated from ΔexsA, ΔexsA : pexsA strains under the inducing (DMEM) growth condition, and NY-4 : pexsA strains under non-inducing (LB-S) growth condition was used as template for the RT reactions. Mock reactions (−), which did not contain reverse transcriptase, were used as controls against genomic contamination of the RNA preparations.
