Table 2.

Nucleotides at cleavage termini generated by PrrC or RloC.

RNA fraction	5′-NMPs	3′-NMPs
43mers	U8 ≫ C = U > Q = I
Band a	U8 ≫ C = U > Q = I	U
∼42mers	G > C = U ≫ X1 = X2
∼52mers	U = X3 = X4 = X5
Band a*	C ≫ U = G = X3 = X4 = X5	U
Band b	U ≫ C = G = X3 = X4 = X5	U
Band c	G ≫ U = C

The indicated labelled RNA products of Fig. 4A were digested by nuclease P1 or RNases T2. The respectively labelled 5′-NMPs and 3′-NMPs released by these digestions were identified by 2D TLC (Fig. 4B) as described in the legend to Fig. 4. The relative amounts of the 5′-NMPs are described as follows: ≫, much greater; >, greater; =, comparable.