Table VII.
Sensitivity of microarray analysis to reduction in slide number. Figures in body of table refer to position of probe in two-way ANOVA gene lists ordered by ascending (diet) P value
| Dye swap | Yes | Yes | Yes | Yes | Yes | No (Cy5 reference) | No (Cy3 reference) | |
|---|---|---|---|---|---|---|---|---|
| Biological replicates | 1–6 | 1–5 | 1–4 | 1–3 | 4–6 | 1–6 | 1–6 | |
| Number of slides per condition | 12 | 10 | 8 | 6 | 6 | 6 | 6 | |
| Gal file identifier | Feature identity | |||||||
| bra_snb_04D02 | Δ5 FAD (full-length EST) | 4 | 5 | 11 | 26 | 1 | 4 | 9 |
| can_D6O_S1B06 | Δ6 FAD ORF (1400 bp) | 5 | 6 | 5 | 8 | 42 | 8 | 8 |
| bra_bfo_03F11 | Δ6 FAD (80% length, EST) | 6 | 10 | 14 | 40 | 3 | 5 | 87 |
| can_D5O_S1B05 | Δ5 FAD ORF (1400 bp) | 7 | 8 | 10 | 21 | 4 | 23 | 7 |
| can_D6D_S1B03 | Δ6 FAD 3′-UTR (384 bp) | 8 | 4 | 4 | — | 213 | 19 | — |
| bra_cbr_B4H01 | Δ5 FAD (partial 3′-UTR, EST) | 10 | 12 | 13 | 18 | 31 | 9 | 26 |
| can_D5D_S1B02 | Δ5 FAD 3′-UTR (881 bp) | 11 | 14 | 16 | 92 | 19 | 12 | 62 |
| can_D6D_S1B04 | Δ6 FAD 3′-UTR (401 bp) | 16 | 34 | 6 | 7 | 451 | 14 | 284 |
| can_D5D_S1B01 | Δ5 FAD 3′-UTR (408 bp) | 25 | 51 | 55 | 94 | 332 | 27 | 60 |
EST, expressed sequence tag; FAD, fatty acyl desaturase; ORF, open reading frame; UTR, untranslated region.