Fig. 3.

Growth characteristics of the T. brucei WT, SKO and DKO cells under various conditions.

A. Growth in HMI9 medium in the presence/absence of drug selection. WT, open circles; SKO plus puromycin, closed circles; DKO plus puromycin and hygromycin, open squares; and DKO without drugs, closed squares. Open triangles show the growth of DKO without drugs in HMI9 medium lacking the thymidine component.

B. Growth in folate-deficient media (FDM) containing 160 μM thymidine. WT, open circles; SKO plus puromycin, closed circles; DKO plus puromycin and hygromycin, open squares; and DKO without thymidine, closed squares. Data are the means of triplicate cultures, where the standard deviations are 5% or less of the value. Symbols on the x-axis indicate that cell densities are below the limits of detection (i.e. < 10³ ml⁻¹). The lines represent the best fits to the equation describing exponential growth as described in Experimental procedures.