Fig. 6.

Northern and Western blot analysis of WT, SKO and DKO cells. Trypanosomes were grown for 24 h in HMI9 medium in the absence (WT and SKO) or presence of thymidine (DKO).

A–C. RNA blots probed with TbDHFR–TS, TbPTR1 and TbINO1 respectively. RNA size markers are indicated to the left of the panels.

D and E. Protein blots of parasite lysates probed with antiserum to PTR1 and BiP respectively. Approximately 20 μg of protein was loaded per lane. Standard size markers are indicated to the right of the panels.