Table 1. Primer sets against rat sequence corresponding to cDNAs identified by subtraction suppressive hybridization.
|
UC6 Library |
DC6 Library |
||
|
Clone |
Rat sequence (5'-3') |
Clone |
Rat sequence (5'-3') |
| 9H |
F: CTCAATGCCGGCTTCAAAGAG |
F5 |
F: CAGCAGTGACCACCAACTGTC |
| R: CGAAGTTCTGCCTGGTAAACG |
R: ATCAAACCAAAACCCTTAGGGG |
||
| 6H |
F: GACTCAGAGATGTAAGATCCC |
G4 |
F: TCTTGTATGAGGACTCCGTGC |
| R: TGTGCAGAGCTCTCTTCAGAC |
R: GGTACAAGAGCCAGCAGCATA |
||
| 4F |
F: ACCCACTAAGCCTCCATTGG |
E2 |
F: CAGAGTCTCCTTTGCCTGAG |
| R: GGTCCTGGAATGAAGCCATG |
R: GGTATGGATCCAACCCACTG |
||
| 2G |
F: TTCCAGCTTGAAAGCCCAGCC |
D2 |
F: CAAGAGGGCCATCTGTACCA |
| R: TGACTCCGGGCTTGTGCGTAA |
R: TTCAGGCACGGATGCAGATG |
||
| 10A |
F: GGCGCCTGCGATTAGTGCTACA |
A7 |
F: GGCAGAGGGCGCTTTAATTTC |
| R: TGATCCTGCCCAGGCCAAAGA |
R: AGTGTCAAGTTCTGCTCCCTG |
||
| F2 |
F: ATCAATTATGAAAGTTTACTGCC |
||
| R: GCTTTTCTTGGATTCATTTGTC |
|||
| D1 |
F: ATGCCTCCATGTGTAGTGCGG |
||
| R: TTTTGGTTGGTTTGCCTAAAA |
|||
| C2 |
F: GAGATGTACTACTGCTCCAAGTG |
||
| R: GTGGTTTATGCTGGCAAAGAG |
|||
| B6 |
F: ATGGAGATGAAGAAGAAGATTAAC |
||
| R: TGTCACTAAGTTCCAACTTCCG |
|||
| B2 |
F: TGAATTTGGCTCAGGAGCTGC |
||
| R: ACTCAGTGAGTCACAGGAGGA |
|||
| G7 |
F: CTGTGGGCTTACAGAGCCAAA |
||
| R: ATGGCTGCTTCCCTCTATAGT |
|||
| C7 |
F: AGGAAGGAAGAGCCTCGTCGT |
||
| R: AGGGTTGCTGCACTGTTACTA |
|||
| A2 |
F: TCCCCTGAACCTGAATGCCTGG |
||
| R: CTCAGCAGACTGCCCGAGGAA |
|||
| S26 |
F: AAGTTTGTCATTCGGAACATT |
||
| R: GATCGATTCCTGACAACCTTG |
Clone denotes the cDNA number in the library, and rat sequence represents forward and reverse primer pairs corresponding to the cDNA. The primers were used to detect gene expression from mRNA extracted from retinal explants or RNA from neonatal rats. DC6 indicates down-regulated by CNTF after 6DIV, UC6 indicates up-regulated by CNTF after 6DIV.