Table 3. Genes isolated by subtractive suppressive hybridization in a DC6 (downregulated by ciliary neurotrophic factor after six days in vitro) library.
|
Clone |
bp |
Blast result |
Symbol |
Accession number |
Homo |
Status |
| GO: apoptosis | ||||||
| D2 |
284 |
Caspase-3 |
Casp3 |
NM_012922 |
37912 |
V |
| A7 |
258 |
Bcl-2 associated transcription factor |
Bclaf |
XM_214967 |
8832 |
A |
| GO: Regulation of gene expression | ||||||
| E2 |
693 |
Cone-Rod homeobox protein |
Crx |
NM_021855 |
467 |
V |
| F2 |
521 |
Bromodomain adjacent zinc finger domain 2b |
Baz2b |
XM_229225 |
8394 |
A |
| G4 |
574 |
t-complex associated testis expressed 1 like |
Tcte1l |
NM_001013228 |
21304 |
no |
| C5 |
369 |
Riken cDNA 1500031M22 |
Dimt1 |
XM_00106535 |
0 7047 |
R |
| D8 |
710 |
C21 ORF66 Isoform D |
N/A |
unknown |
9604 |
R |
| GO: Protein modification | ||||||
| D1 |
465 |
Ste-20 related kinase |
Spak |
AF099990 |
22739 |
A |
| C2 |
445 |
Ubiquitin Specific Peptidase 32 |
Usp32 |
NM_220798 |
13066 |
A |
| B6 |
688 |
Acidic nuclear phosphoprotein 32 family member E |
Anp32e |
NM_001013200 |
41519 |
A |
| B2 |
290 |
Protein tyrosine phosphatase interracting protein |
Ptpip51 |
BC082081 |
34926 |
A |
| G7 |
103 |
6 Epm2a interracting protein |
Epm2aip |
XM_236659 |
8875 |
A |
| D4 |
240 |
Riken cDNA 5330408N05 gene |
N/A |
XM_23000 |
36420 |
R |
| GO: Protein localization | ||||||
| C7 |
490 |
Translocation protein 1 |
Tloc1 |
BC099207 |
2449 |
A |
| GO: signal transduction | ||||||
| A2 |
501 |
ADP-ribosylation factor 4 |
Arf4 |
NM_024151 |
55593 |
A |
| F11 |
350 |
Homolog to TBC1 Domain family mb 15 |
Tbc1d15 |
XM_345825 |
11249 |
R |
| GO: Cell Ion Homeostasis | ||||||
| F5 | 670 | Iron Responsive Element Binding protein 2 | Ireb2 | NM_022863 | 11280 | no |
DC6 subtraction suppressive hybridization library was generated using RNA from control explants as driver and the RNA from six days in vitro (6DIV)-ciliary neurotrophic factor treated explants as tester. Clone represents the cDNA number in the library; bp denotes the size in base pair of the cDNA; blast result indicates the published sequence identical to the clone sequence Symbol, Accession number, and Homologene refer to the available on-line datas for the published sequence. Status represents the cDNA characterization in this study: A for analyzed, R for rejected, no for gene that didn't exhibit gene expression variation, and V for gene validating the library. cDNA were classified accordingly to their gene ontology (GO).