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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Bioorg Med Chem Lett. 2008 Jul 18;18(22):5916–5919. doi: 10.1016/j.bmcl.2008.07.062

Figure 5.

Figure 5

Irreversible EGFR inhibitors inhibit autophosphorylation of overexpressed Bmx in LNCaP cells, whereas reversible inhibitors show no effect. (a) Immunoprecipitated Flag-Bmx was pre-incubated with different drugs at 4°C for 2 hrs, and in vitro kinase assay using 32P-ATP was performed. (b) In vitro kinase assay using Flag-Bmx immunopurified from the cells that were pre-treated with drugs for 4 hrs. Autophosphorylation was examined by using both radiography and phosphotyrosine immunoblot analysis.