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. Author manuscript; available in PMC: 2008 Dec 29.
Published in final edited form as: Chem Biol Drug Des. 2006 Mar;67(3):230–237. doi: 10.1111/j.1747-0285.2006.00361.x

Figure 3. Effects of Arg mutations in the Abl SH2 domain.

Figure 3

Basic residues near the Abl SH2 domain binding site were mutated conservatively. The interactions of C4-PtdIns(4,5)P2 and pTyr amino acid at final concentrations of 0.8 mM and 3.2-4 mM, respectively, were analyzed by HSQC NMR titrations and compared to the wild type (see Figures 1 and 2) for each of three different mutants Arg36Lys (A, D), Arg45Gln (B, E) and Arg59Gln (C, F). HSQC spectra were recorded and overlaid to visualize the chemical shift changes which were plotted by residue position in the histograms.