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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Methods. 2008 Dec;5(12):1005–1010. doi: 10.1038/nmeth.1270

Figure 3. A-tailing, ligation and size selection.

Figure 3

GC plots before (a) and after (b) optimisation of gel extraction. The figures show the total area in which reads with a particular GC content are distributed, with the mean and standard deviation. The greater width of shaded area in plot a) indicates a wider dispersion of coverage for all values of GC content for which sequences were obtained.

Agilent traces Bioanalyzer 2100 traces for two suboptimal libraries c) 60bp insert library, with optimised PCR, d) the same 60bp library with excess DNA in PCR e) 200bp insert library, showing shoulder of small fragments.

Insert size distribution from sequenced human DNA using f) the standard and g) modified paired end library prep protocols