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Generation of THYFP/+ knock-in mES cells and mice. (A) Schematic of targeting strategy used to insert the gene for YFP into the TH gene locus by homologous recombination. Southern blot digestion sites and PCR primers for genotyping are indicated. (B) Southern blot analysis of genomic DNA after digestion with SpeI confirmed homologous recombination and in vitro Neo excision. (C) PCR of mouse tail DNA confirmed heterozygous TH/YFP genotype and excision of Neo.
