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. Author manuscript; available in PMC: 2008 Dec 29.
Published in final edited form as: Neuroscience. 2006 Jul 31;142(2):343–354. doi: 10.1016/j.neuroscience.2006.06.032

Fig. 4.

Fig. 4

DNaseI hypersensitivity assay of the TH gene in C6 glioma cells and PC12 cells. (A) Southern blot of chromosomal DNA from TH-negative C6 glioma cells and TH-positive PC12 cells treated with increasing concentrations of DNaseI. DNA was digested with EcoRI and probed using an EcoRI-PstI rat cDNA fragment that spans from nucleotides 117–1137 bp (exons 2–11) within the TH coding region. (B) Schematic of hypersensitivity sites identified in the TH gene. Sites HS3-6 fall within the region deleted in the reporter system. (C) Schematic map of the first exon, first intron and second exon of the mouse, rat and human TH genes.