Figure 6.

Supernatants were collected from splenocytes, of 24 week-old mice that were prenatally exposed to 0.0, 2.5 and 5.0 µg/kg TCDD, cultured for 48 hr with Con A (10 µg/mL). The levels of IL-2, IL-4, IL-10, IL-12 and INF-γ were determined using commercially-available murine cytokine ELISA kits. The data are presented in a scatterplot analysis with mean bars (n = 5 mice/treatment/gender,*= p ≤ 0.05, Dunnett’s t-test).